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KMID : 0903520050480040331
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2005 Volume.48 No. 4 p.331 ~ p.334
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Comparison of the Efficiency from Raw and Processed Corns by Five Different DNA Extraction Methods
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Lee Hun-Hee
Song Hee-Sung
Kim Jae-Hwan
Lee Woo-Young
Lee Sun-Ho
Park Sun-Hee
Park Hye-Kyung
Kim Hae-Yeong
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Abstract
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In this study, the effects of five extraction methods for raw and processed corns were compared with respect to the integrity, yields and quality of DNA extracted from them and the results were assessed by PCR analysis. From the comparison of five extraction methods, DNA integrity showed a similar pattern. Amounts of genomic DNA obtained from the five extraction methods varies from 0.25 μg to 234 μg per 1 g sample. The DNA yield extracted with CTAB method and DNeasy Plant Maxi kit is greater than that obtained from other extraction methods. These results would be applicable for the selection of an adequate extraction method for specific samples.
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KEYWORD
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DNA extraction, GM Maize, PCR
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